Process for transforming Gramineae and the products thereof
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United States Patent and Trademark Office
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A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and inoculating the wound with vir.sup.+ Agrobacterium tumefaciens. Also, this same method wherein the vir.sup.+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment of T-DNA, and Gramineae derived from each of these pollen grains. There are also provided a transformed Gramineae plant, a transformed Gramineae plant derived from a seedling infected with vir.sup.+ Agrobacterium tumefaciens, a transformed Gramineae plant derived from a seedling infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA and a Gramineae plant whose cells contain a segment of T-DNA. Finally, there are provided transformed Gramineae derived from seedlings infected with vir.sup.+ Agrobacterium tumefaciens and transformed Gramineae derived from seedlings infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA.
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800/294, 435/469, 800/300, 800/320, 800/320.1, 800/320.3