System for simultaneous isomerization and fermentation of sugars

Methods and systems for the isomerization and fermentation of xylose and hexose sugars using an immobilized enzyme system capable of sustaining two different pH microenvironments in a single vessel are disclosed. Bilayer particles are dispersed in a mixture comprising an ionic borate source and xylose. The bilayer particles have a first region with a first enzymatic activity comprising xylose isomerase and a pH of 6 or above, and a second region having a second enzymatic activity at an acidic pH.
Download pdf
Abstract/Description: Methods and systems for the isomerization and fermentation of xylose and hexose sugars using an immobilized enzyme system capable of sustaining two different pH microenvironments in a single vessel are disclosed. Bilayer particles are dispersed in a mixture comprising an ionic borate source and xylose. The bilayer particles have a first region with a first enzymatic activity comprising xylose isomerase and a pH of 6 or above, and a second region having a second enzymatic activity at an acidic pH.
Subject(s): C12N11/00
C12M1/00
C12M1/40
C12N1/16
C12N1/18
C12N11/18
C12P7/06
C12P7/14
Date Issued: 2018-01-02
Title: System for simultaneous isomerization and fermentation of sugars.
Name(s): The University of Toledo, assignee
Varanasi, Sasidhar, inventor
Rao, Kripa, inventor
Relue, Patricia Ann, inventor
Yuan, Dawei, inventor
Type of Resource: text
Genre: patent
Publisher: United States Patent and Trademark Office
Other Date: Date Filed: 2016-12-22
Date Issued: 2018-01-02
Physical Form: application/pdf
Extent: 27 p.
Abstract/Description: Methods and systems for the isomerization and fermentation of xylose and hexose sugars using an immobilized enzyme system capable of sustaining two different pH microenvironments in a single vessel are disclosed. Bilayer particles are dispersed in a mixture comprising an ionic borate source and xylose. The bilayer particles have a first region with a first enzymatic activity comprising xylose isomerase and a pH of 6 or above, and a second region having a second enzymatic activity at an acidic pH.
Identifier(s): utpatents-US9856445 (IID)
Patent No.: US9856445 (patno)
Appl. No.: 15/388443 (appno)
Subject(s): C12N11/00
C12M1/00
C12M1/40
C12N1/16
C12N1/18
C12N11/18
C12P7/06
C12P7/14
Held by: United States Patent and Trademark Office (USPTO) Public Search Facility
Location: University of Toledo Digital Repository
Rights Statement: No Copyright - United States
In Collections: