Process for transforming gladiolus

A method of producing a transformed Gladiolus plant comprising: removing a piece of tissue from a corm; inoculating the tissue with vir+ Agrobacterium tumefaciens; incubating the inoculated tissue until a tumor forms; culturing at least a portion of the tumor in hormone-free medium until a cormel forms; and growing the cormel to produce the transformed plant. Also, methods of producing a transformed Gladiolus corm or seed comprising growing a transformed Gladiolus plant, prepared as just described, until the corm or seed is formed. Finally, transformed Gladiolus plants, corms and seeds.
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Abstract/Description: A method of producing a transformed Gladiolus plant comprising: removing a piece of tissue from a corm; inoculating the tissue with vir+ Agrobacterium tumefaciens; incubating the inoculated tissue until a tumor forms; culturing at least a portion of the tumor in hormone-free medium until a cormel forms; and growing the cormel to produce the transformed plant. Also, methods of producing a transformed Gladiolus corm or seed comprising growing a transformed Gladiolus plant, prepared as just described, until the corm or seed is formed. Finally, transformed Gladiolus plants, corms and seeds.
Subject(s): 800/294
800/298
800/323
Date Issued: 1994-08-23