Process for transforming gramineae and the products thereof

A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and inoculating the wound with vir+ Agrobacterium tumefaciens. Also, this same method wherein the vir+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment of T-DNA, and Gramineae derived from each of these pollen grains. There are also provided a transformed Gramineae plant, a transformed Gramineae plant derived from a seedling infected with vir+ Agrobacterium tumefaciens, a transformed Gramineae plant derived from a seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA and a Gramineae plant whose cells contain a segment of T-DNA. Finally, there are provided transformed Gramineae derived from seedlings infected with vir+ Agrobacterium tumefaciens and transformed Gramineae derived from seedlings infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA.
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Abstract/Description: A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and inoculating the wound with vir+ Agrobacterium tumefaciens. Also, this same method wherein the vir+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment of T-DNA, and Gramineae derived from each of these pollen grains. There are also provided a transformed Gramineae plant, a transformed Gramineae plant derived from a seedling infected with vir+ Agrobacterium tumefaciens, a transformed Gramineae plant derived from a seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA and a Gramineae plant whose cells contain a segment of T-DNA. Finally, there are provided transformed Gramineae derived from seedlings infected with vir+ Agrobacterium tumefaciens and transformed Gramineae derived from seedlings infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA.
Subject(s): 800/294
435/252.2
435/320.1
Date Issued: 1993-02-16
Title: Process for transforming gramineae and the products thereof.
Name(s): The University of Toledo, assignee
Goldman, Stephen L., inventor
Graves, Anne C. F., inventor
Type of Resource: text
Genre: patent
Publisher: United States Patent and Trademark Office
Other Date: Date Filed: 1989-11-13
Date Issued: 1993-02-16
Physical Form: application/pdf
Extent: 24 p.
Abstract/Description: A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and inoculating the wound with vir+ Agrobacterium tumefaciens. Also, this same method wherein the vir+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment of T-DNA, and Gramineae derived from each of these pollen grains. There are also provided a transformed Gramineae plant, a transformed Gramineae plant derived from a seedling infected with vir+ Agrobacterium tumefaciens, a transformed Gramineae plant derived from a seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA and a Gramineae plant whose cells contain a segment of T-DNA. Finally, there are provided transformed Gramineae derived from seedlings infected with vir+ Agrobacterium tumefaciens and transformed Gramineae derived from seedlings infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA.
Identifier(s): utpatents-US5187073 (IID)
Patent No.: US5187073 (patno)
Appl. No.: 07/436187 (appno)
Subject(s): 800/294
435/252.2
435/320.1
Held by: United States Patent and Trademark Office (USPTO) Public Search Facility
Location: University of Toledo Digital Repository
Rights Statement: No Copyright - United States
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