utpatents-US5177010

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Abstract/Description: A method of producing transformed corn comprising making a wound in a corn seedling in an area of the corn seedling containing rapidly dividing cells and inoculating the wound with vir+ Agrobacterium tumefaciens. Also, this same method wherein the vir+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed corn pollen grain, a corn pollen grain produced by a plant grown from a seedling infected with vir+ A. tumefaciens, a corn pollen grain produced by a plant grown from a seedling infected with vir+ A. tumefaciens containing a vector comprising genetrically-engineered T-DNA, a corn pollen grain whose cells contain a segment of T-DNA, and corn derived from each of these pollen grains. There are also provided a transformed corn plant, a transformed corn plant derived from a corn seedling infected with vir+ Agrobacterium tumefaciens, a transformed corn plant derived from a corn seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, and a corn plant whose cells contain a segment of T-DNA. Finally, there are provided transformed corn derived from a corn seeding infected with vir+ Agrobacterium tumefaciens, and transformed corn derived from a corn seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA.
Subject(s): 800/294
435/252.2
435/252.3
435/320.1
Date Issued: 1993-01-05