Tissue culture system

A tissue culture system for zero gravity high-density cultivation of eucaryotic cells requiring attachment to a substratum in order to differentiate is disclosed. A major component of the system is a cylindrical bottle filled with culture medium and having mounted therein a rotatable shaft. The shaft includes a plurality of collagen-treated discs projecting therefrom and at an angle to the shaft to provide the necessary attachment substratum and to impel the culture medium as the shaft is rotated. Means are provided for initial stocking of the bottle with the culture cells and for continuously supplying fresh medium to and removing waste medium from the bottle.
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Abstract/Description: A tissue culture system for zero gravity high-density cultivation of eucaryotic cells requiring attachment to a substratum in order to differentiate is disclosed. A major component of the system is a cylindrical bottle filled with culture medium and having mounted therein a rotatable shaft. The shaft includes a plurality of collagen-treated discs projecting therefrom and at an angle to the shaft to provide the necessary attachment substratum and to impel the culture medium as the shaft is rotated. Means are provided for initial stocking of the bottle with the culture cells and for continuously supplying fresh medium to and removing waste medium from the bottle.
Subject(s): 435/299.1
Date Issued: 1980-06-17
Title: Tissue culture system.
Name(s): The University of Toledo, assignee
Lee, Harold H., inventor
Type of Resource: text
Genre: patent
Publisher: United States Patent and Trademark Office
Other Date: Date Filed: 1978-09-21
Date Issued: 1980-06-17
Physical Form: application/pdf
Extent: 5 p.
Abstract/Description: A tissue culture system for zero gravity high-density cultivation of eucaryotic cells requiring attachment to a substratum in order to differentiate is disclosed. A major component of the system is a cylindrical bottle filled with culture medium and having mounted therein a rotatable shaft. The shaft includes a plurality of collagen-treated discs projecting therefrom and at an angle to the shaft to provide the necessary attachment substratum and to impel the culture medium as the shaft is rotated. Means are provided for initial stocking of the bottle with the culture cells and for continuously supplying fresh medium to and removing waste medium from the bottle.
Identifier(s): utpatents-US4208483 (IID)
Patent No.: US4208483 (patno)
Appl. No.: 05/944267 (appno)
Subject(s): 435/299.1
Held by: United States Patent and Trademark Office (USPTO) Public Search Facility
Location: University of Toledo Digital Repository
Rights Statement: No Copyright - United States
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