Methods for standardized sequencing of nucleic acids and uses thereof

Methods for controlling for non-systematic error in an amplification-based next generation sequencing (NGS) library preparation are described, which method includes using an internal amplification control (IAC) sharing identical priming sites to a native nucleic acid target template of interest in a NGS library preparation.
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Abstract/Description: Methods for controlling for non-systematic error in an amplification-based next generation sequencing (NGS) library preparation are described, which method includes using an internal amplification control (IAC) sharing identical priming sites to a native nucleic acid target template of interest in a NGS library preparation.
Subject(s): C40B20/04
C12Q1/6806
C12Q1/6851
C12Q1/6869
Date Issued: 2022-04-26
Title: Methods for standardized sequencing of nucleic acids and uses thereof.
Name(s): Willey, James C., inventor
Blomquist, Thomas, inventor
Crawford, Erin, inventor
The University of Toledo, assignee
Type of Resource: text
Genre: patent
Date Issued: 2022-04-26
Other Date: Date Filed: 2018-03-01
Publisher: United States Patent and Trademark Office
Physical Form: application/pdf
Extent: 63 p.
Abstract/Description: Methods for controlling for non-systematic error in an amplification-based next generation sequencing (NGS) library preparation are described, which method includes using an internal amplification control (IAC) sharing identical priming sites to a native nucleic acid target template of interest in a NGS library preparation.
Identifier(s): utpatents-US11312987 (IID)
Patent No.: US11312987 (patno)
Appl. No.: 15/909525 (appno)
Subject(s): C40B20/04
C12Q1/6806
C12Q1/6851
C12Q1/6869
Held by: United States Patent and Trademark Office (USPTO) Public Search Facility
Location: University of Toledo Digital Repository
Rights Statement: No Copyright - United States
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