Methods for standardized sequencing of nucleic acids and uses thereof

Methods for controlling for non-systematic error in an amplification-based next generation sequencing (NGS) library preparation are described, which method includes using an internal amplification control (IAC) sharing identical priming sites to a native nucleic acid target template of interest in a NGS library preparation.
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Abstract/Description: Methods for controlling for non-systematic error in an amplification-based next generation sequencing (NGS) library preparation are described, which method includes using an internal amplification control (IAC) sharing identical priming sites to a native nucleic acid target template of interest in a NGS library preparation.
Subject(s): C40B20/04
C12Q1/6806
C12Q1/6851
C12Q1/6869
Date Issued: 2022-04-26