Document Type

Patent

Abstract

Described herein are materials, methods, and kits enabling accurate and reproducible two-color reverse-transcription real-time quantitative PCR (RT-qPCR) for quality-controlled molecular diagnostic testing of samples that may contain degraded RNA. In certain aspects described herein are materials, methods, and kits for use in the molecular diagnostic testing of lung cancer in FFPE samples and/or fresh-frozen samples. Also described herein are materials and methods to control for inter-experimental variation occurring during two-color RT-qPCR amplification arising from variation in fluorescence specific activity, use of different thermocyclers, and inter-laboratory differences.

Patent Country

United States

Patent Number

US9528161

Publication Date

12-27-2016

Assignee(s)

The University of Toledo

Application Number

14/619285

Filing Date

2-10-2015

Primary Class

C12Q1/68

Secondary Class

G06F19/24

Rights

These materials are free of copyright restrictions and are in the public domain within the United States only. The USPTO reserves the right to assert copyright protection internationally.

Institution

The University of Toledo

Repository

University of Toledo Digital Libraries

Digital Publisher

Digital Initiatives

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