Abstract

A tissue culture system for zero gravity high-density cultivation of eucaryotic cells requiring attachment to a substratum in order to differentiate is disclosed. A major component of the system is a cylindrical bottle filled with culture medium and having mounted therein a rotatable shaft. The shaft includes a plurality of collagen-treated discs projecting therefrom and at an angle to the shaft to provide the necessary attachment substratum and to impel the culture medium as the shaft is rotated. Means are provided for initial stocking of the bottle with the culture cells and for continuously supplying fresh medium to and removing waste medium from the bottle.

Document Type

Patent

Primary Class

435/299.1

Application Number

05/944267

Filing Date

September 1978

Patent Number

US4208483

Assignee(s)

The University of Toledo

Publication Date

6-17-1980

Patent Country

US

Language

en_US

Institution

The University of Toledo

Repository

University of Toledo Libraries

Digital Publisher

Digital Initiatives

Rights

These materials are free of copyright restrictions and are in the public domain within the United States only. The USPTO reserves the right to assert copyright protection internationally.

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Rights Statement

Rights Statement

No Copyright - United States. URI: http://rightsstatements.org/vocab/NoC-US/1.0/
The organization that has made the Item available believes that the Item is in the Public Domain under the laws of the United States, but a determination was not made as to its copyright status under the copyright laws of other countries. The Item may not be in the Public Domain under the laws of other countries. Please refer to the organization that has made the Item available for more information.