Document Type

Patent

Abstract

A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and inoculating the wound with vir+ Agrobacterium tumefaciens. Also, this same method wherein the vir+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment of T-DNA, and Gramineae derived from each of these pollen grains. There are also provided a transformed Gramineae plant, a transformed Gramineae plant derived from a seedling infected with vir+ Agrobacterium tumefaciens, a transformed Gramineae plant derived from a seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA and a Gramineae plant whose cells contain a segment of T-DNA. Finally, there are provided transformed Gramineae derived from seedlings infected with vir+ Agrobacterium tumefaciens and transformed Gramineae derived from seedlings infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA.

Patent Country

US

Patent Number

US5187073

Publication Date

2-16-1993

Assignee(s)

The University of Toledo

Application Number

07/436187

Filing Date

November 1989

Primary Class

800/294

Secondary Class

435/252.2; 435/320.1

Rights

These materials are free of copyright restrictions and are in the public domain within the United States only. The USPTO reserves the right to assert copyright protection internationally.

Institution

The University of Toledo

Repository

University of Toledo Libraries

Digital Publisher

Digital Initiatives

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